After her graduation in 1982 Roos worked as an architect, project manager and consultant. Until 1990 she worked on new construction development and urban renewal projects, both utilitarian as well as housing projects. After a period of 3 years abroad (Colombia), she worked as a development manager in urban renewal and area development projects, serving real estate companies and municipal corporations. Since 2004 she has been working as an interim manager and process manager in area development. Some of her projects are specifically aimed at housing and real estate in healthcare. Research and advice have always been important elements of her projects. In addition, teaching has often been part of her working career: she taught Architecture and Urbanism as a guest lecturer in Cartagena, Colombia; she has been teaching Area Development at Delft University of Technology and Quality management at the Architecture Faculty in Sucre, Bolivia.
Urban planning and urban heritage are important disciplines for her. Roos has been Board member of the BNSP (Association of Dutch Urban Designers and Planners) and she is member of the Supervisory Commission of the study of Heritage and Area Development at the Hogeschool of Utrecht.
She expanded her professional profile with respect to the Latin American city and culture through a master in Latin American studies at the University of Leiden. In 2017, she completed her master thesis titled: "The transformation of the city in Latin America and the development of tourism. Urban and tourist development of Cartagena ".
Roos Stolker participates as a dual PhD candidate at Leiden University Dual PhD Centre. Working title: 'Development perspective on urban heritage. Transformation of the Latin American city'.